Differentiation of embryonic stem cells into lung-like cells using lung-derived matrix sheets

Tomotaka Kitamura; Masayasu Misu; Masahide Yoshikawa; Yukiteru Ouji

doi:10.1016/j.bbrc.2023.149197

Differentiation of embryonic stem cells into lung-like cells using lung-derived matrix sheets

Biochem Biophys Res Commun. 2023 Dec 17:686:149197. doi: 10.1016/j.bbrc.2023.149197. Epub 2023 Oct 31.

Authors

Tomotaka Kitamura¹, Masayasu Misu¹, Masahide Yoshikawa¹, Yukiteru Ouji²

Affiliations

¹ Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan.
² Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan. Electronic address: oujix@naramed-u.ac.jp.

PMID: 37924668
DOI: 10.1016/j.bbrc.2023.149197

Abstract

Various extracellular matrix (ECM) in the lungs regulate tissue development and homeostasis, as well as provide support for cell structures. However, few studies regarding the effects of lung cell differentiation using lung-derived ECM (LM) alone have been reported. The present study investigated the capability of lung-derived matrix sheets (LMSs) to induce lung cell differentiation using mouse embryonic stem (ES) cells. Expressions of lung-related cell markers were significantly upregulated in ES-derived embryoid bodies (EBs) cultured on an LMS for two weeks. Moreover, immunohistochemical analysis of EBs grown on LMSs revealed differentiation of various lung-related cells. These results suggest that an LMS can be used to promote differentiation of stem cells into lung cells.

Keywords: Differentiation; Extracellular matrix; Matrix sheets; Stem cell; lung.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / physiology
Cells, Cultured
Embryoid Bodies*
Embryonic Stem Cells*
Lung
Mice