Exosomes derived from adipose stem cells in combination with hyaluronic acid promote diabetic wound healing

Huldani Huldani; Iuliia A Kozlitina; Mesfer Alshahrani; Hamid Mahmood Abdullah Daabo; Sami G Almalki; Khulood H Oudaha; Ahmed Hussien Alawadi; Ali Alsalamy; S K Joshi; Yasser Fakri Mustafa

doi:10.1016/j.tice.2023.102252

Exosomes derived from adipose stem cells in combination with hyaluronic acid promote diabetic wound healing

Tissue Cell. 2023 Dec:85:102252. doi: 10.1016/j.tice.2023.102252. Epub 2023 Oct 21.

Affiliations

¹ Department of Physiology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia. Electronic address: huldani2020@gmail.com.
² Department of Pediatric, Preventive Dentistry and Orthodontics, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
³ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
⁴ Department of Pharmacy, Duhok Polytechnic University, Duhok, Iraq.
⁵ Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia.
⁶ Pharmaceutical Chemistry Department, College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq.
⁷ College of technical engineering, Islamic University, Najaf, Iraq; College of technical engineering, Islamic University of Al Diwaniyah, Iraq; College of technical engineering, Islamic University of Babylon, Iraq.
⁸ College of technical engineering, Imam Ja'afar Al-Sadiq University, Al-Muthanna 66002, Iraq.
⁹ Department of Mechanical Engineering, Uttaranchal Institute of Technology, Uttaranchal University, Dehradun 248007, India.
¹⁰ Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul 41001, Iraq.

PMID: 37922674
DOI: 10.1016/j.tice.2023.102252

Abstract

Diabetic wound is one of the main challenges in dermatology. Although stem cell-based treatment has therapeutic benefits in wound repair, the clinical application is still limited. Herein we investigated whether adipose stem cells -derived exosomes (Exo) loaded on hyaluronic acid (HA) could promote healing in diabetic rats. Sixty diabetic rats were randomly planned into the control group, Exo group, HA group, and HA+Exo group. On days 7, 14, and 21, five rats from each group were sampled for stereological, molecular, and tensiometrical assessments. Our results indicated that the wound closure rate, the total volumes of new epidermis and dermis, the numerical densities of fibroblasts, the length density blood vessels, collagen density as well as tensiometrical parameters of the healed wounds were significantly higher in the treated groups than in the control group, and these changes were more obvious in the HA+Exo ones. Furthermore, the expression of TGF-β and VEGF genes were meaningfully upregulated in all treated groups compared to the control group and were greater in the HA+Exo group. This is while expression of TNF-α and IL-1β, as well as numerical densities of neutrophils decreased more considerably in the HA+Exo group in comparison to the other groups. Generally, it was found that using both HA injection and exosomes has more effect on diabetic wound healing.

Keywords: Adipose-derived stem cells; Diabetic wound; Exosomes; Hyaluronic acid; Wound healing.

MeSH terms

Animals
Diabetes Mellitus, Experimental* / metabolism
Exosomes* / metabolism
Hyaluronic Acid / pharmacology
Rats
Stem Cells
Wound Healing

Substances

Hyaluronic Acid