Effects of pyrrolopyrimidine derivatives on cancer cells cultured in vitro and potential mechanism

Naunyn Schmiedebergs Arch Pharmacol. 2024 May;397(5):3169-3177. doi: 10.1007/s00210-023-02799-6. Epub 2023 Oct 28.

Abstract

In this study, the anticancer activities of some pyrrolopyrimidine derivatives were evaluated. Compound 3 is the most cytotoxic compound on MCF-7 cancer cells with an IC50 value of 23.42 µM. Also, compound 3 induced apoptosis and the ROS(+) cell population in MCF-7 cells. Moreover, it significantly reduced MMP-9 activity, having 42.16 ± 5.10% and 58.28 ± 1.96% inhibitory activities at 10 µM and 50 µM concentrations, respectively. Molecular docking results supported the activity, showing key hydrogen bonds with the binding site of MMP-9. Therefore, compound 3 might be a lead compound for the development of potent MMP-9 inhibitors.

Keywords: Anticancer; MMP-9 inhibitor activity; Oxidative stress; Pyrrolopyrimidine derivatives.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents* / chemistry
  • Antineoplastic Agents* / pharmacology
  • Apoptosis* / drug effects
  • Cell Line, Tumor
  • Humans
  • MCF-7 Cells
  • Matrix Metalloproteinase 9* / metabolism
  • Molecular Docking Simulation*
  • Pyrimidines* / chemistry
  • Pyrimidines* / pharmacology
  • Pyrroles* / chemistry
  • Pyrroles* / pharmacology
  • Reactive Oxygen Species* / metabolism

Substances

  • Pyrimidines
  • Pyrroles
  • pyrrolopyrimidine
  • Antineoplastic Agents
  • Reactive Oxygen Species
  • Matrix Metalloproteinase 9
  • MMP9 protein, human