Mind your tag in single-molecule measurements

Raquel Merino Urteaga; Taekjip Ha

doi:10.1016/j.crmeth.2023.100623

Mind your tag in single-molecule measurements

Cell Rep Methods. 2023 Oct 23;3(10):100623. doi: 10.1016/j.crmeth.2023.100623.

Authors

Raquel Merino Urteaga¹, Taekjip Ha²

Affiliations

¹ Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA; Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
² Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, USA; Howard Hughes Medical Institute, Boston, MA, USA. Electronic address: taekjip.ha@childrens.harvard.edu.

Abstract

In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of other tags on DNA-binding proteins.

Publication types

Comment

MeSH terms

Bacillus subtilis / genetics
DNA* / metabolism
DNA-Binding Proteins / genetics
Lysine* / metabolism

Substances

Lysine
DNA
DNA-Binding Proteins