Characterization of VIM-71, a novel VIM-type metallo-β-lactamase variant encoded by an integrative and conjugative element recovered from a Vibrio alginolyticus strain in China

Zhiwei Zheng; Qipeng Cheng; Lianwei Ye; Yating Xu; Sheng Chen

doi:10.1016/j.micres.2023.127532

Characterization of VIM-71, a novel VIM-type metallo-β-lactamase variant encoded by an integrative and conjugative element recovered from a Vibrio alginolyticus strain in China

Microbiol Res. 2024 Jan:278:127532. doi: 10.1016/j.micres.2023.127532. Epub 2023 Oct 19.

Authors

Zhiwei Zheng¹, Qipeng Cheng², Lianwei Ye³, Yating Xu³, Sheng Chen⁴

Affiliations

¹ The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PR China; State Key Lab for Chemical Biology and Drug Discovery, and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
² College of Life Sciences, Anhui Normal University, Wuhu, PR China; State Key Lab for Chemical Biology and Drug Discovery, and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
³ State Key Lab for Chemical Biology and Drug Discovery, and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
⁴ The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PR China; State Key Lab for Chemical Biology and Drug Discovery, and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: sheng.chen@polyu.edu.hk.

PMID: 37879253
DOI: 10.1016/j.micres.2023.127532

Abstract

A novel VIM-type metallo-β-lactamase variant, VIM-71, which is encoded by a multidrug-resistant Vibrio alginolyticus strain recovered from a shrimp sample in China, was identified. Compared to VIM-1, VIM-71 differs in 22 amino acid positions based on the primary protein sequence and confers a similar resistance profile to penicillins, but the level of resistance to carbapenems encoded by this enzyme was lower than that of VIM-1. The bla_VIM-71 gene was found located in an integrative and conjugative element of the SXT/R391 family in the chromosome. These findings implied that genetic elements that encode clinically important carbapenemases continue to evolve in Vibrio spp.

Keywords: Carbapenemase; Integrative and conjugative element; Novel VIM-like; Vibrio alginolyticus; WGS.

MeSH terms

Anti-Bacterial Agents / pharmacology
Carbapenems / metabolism
China
Microbial Sensitivity Tests
Penicillins
Vibrio alginolyticus* / genetics
Vibrio alginolyticus* / metabolism
beta-Lactamases* / genetics
beta-Lactamases* / metabolism

Substances

beta-Lactamases
Carbapenems
Penicillins
Anti-Bacterial Agents