Evaluation of packaging capacity at the genomic 2C/3A junction region in Porcine enterovirus G

Shiting Huang; Xue Mi; Tongwei Ren; Daling Hong; Qiuying Qin; Meijing Long; Yifeng Qin; Ying Chen; Zuzhang Wei; Weijian Huang; Kang Ouyang

doi:10.1016/j.virol.2023.109899

Evaluation of packaging capacity at the genomic 2C/3A junction region in Porcine enterovirus G

Virology. 2023 Nov:588:109899. doi: 10.1016/j.virol.2023.109899. Epub 2023 Oct 13.

Authors

Affiliations

¹ Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China.
² Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530005, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, 530005, China.
³ Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530005, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, 530005, China. Electronic address: ouyangkang@gxu.edu.cn.

PMID: 37862828
DOI: 10.1016/j.virol.2023.109899

Abstract

Porcine enterovirus G (EV-G) is endogenous to most pig farming countries worldwide. Reports that a papain-like protease (PLP) gene has been naturally inserted into the 2C/3A junction region of the EV-G genome, has increased the potential public health threats from this virus. We constructed a full-length infectious cDNA clone of EV-G, CH/17GXQZ/2017, in order to determine the packaging capacity at the 2C/3A insertion site. Subsequently, recombinants viruses containing the coding tags, GFP, iLOV and His at the 2C/3A junction region, were synthesized. The infectious virus was successfully rescued only with the insertion of the His-tag, which displayed similar virological and molecular properties to its parental strain. This study determined the packaging capacity of the 2C/3A insertion site, and it provides a practical tool for studying the functions and pathogenic mechanisms of EV-G in pigs.

Keywords: 2C/3A junction; Picornavirus; Porcine enterovirus G; Reverse genetics; Viral genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Enteroviruses, Porcine* / genetics
Genome, Viral
Genomics
Swine