Ciliated epithelia are common in various human organs, indeed across many species, and their physiological functions are vital. A number of diseases, of genetic, degenerative, or infectious nature, compromise motile cilia function and lead to severe downstream consequences. Culture of ciliated tissues is a common research approach. We focus here on the video microscopy and analysis pipelines developed over the last few years to phenotype ciliary beating in lung cells, specifically to extract: cilia coverage; ciliary beat frequency distributions; the scale for ciliary dynamical coordination; and cilia beat waveform.
Keywords: Cilia dynamics; Motile cilia phenotyping; Video analysis ciliated tissues.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.