Micronized Acellular Matrix Biomaterial Leverages Eosinophils for Postinfarct Cardiac Repair

Vishnu Vasanthan; Ali Fatehi Hassanabad; Darrell Belke; Guoqi Teng; Carmina Albertine Isidoro; Devjyoti Dutta; Jeannine Turnbull; Justin F Deniset; Paul W M Fedak

doi:10.1016/j.jacbts.2023.01.012

Micronized Acellular Matrix Biomaterial Leverages Eosinophils for Postinfarct Cardiac Repair

JACC Basic Transl Sci. 2023 Mar 22;8(8):939-954. doi: 10.1016/j.jacbts.2023.01.012. eCollection 2023 Aug.

Authors

Affiliations

¹ Department of Cardiac Sciences, Libin Cardiovascular Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
² Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada.

Abstract

After ischemic injury, immune cells mediate maladaptive cardiac remodeling. Extracellular matrix biomaterials may redirect inflammation toward repair. Pericardial fluid contains pro-reparative immune cells, potentially leverageable by biomaterials. Herein, we explore how pericardial delivery of a micronized extracellular matrix biomaterial affects cardiac healing. In noninfarcted mice, pericardial delivery increases pericardial and myocardial eosinophil counts. This response is sustained after myocardial infarction, stimulating an interleukin 4 rich milieu. Ultimately, the biomaterial improves postinfarct vascularization and cardiac function; and eosinophil-knockout negates these benefits. For the first time, to our knowledge, we demonstrate the therapeutic potential of pericardial biomaterial delivery and the eosinophil's critical role in biomaterial-mediated postinfarct repair.

Keywords: biomaterial; cardiac repair; eosinophil; extracellular matrix; pericardial space.