Can Superoxide Anions Predict the Malignant Potential of Carotid body Tumor? - A Pilot Study

S Kajal; Arvind Kumar Kairo; Javed Ahsan Quadri; Saba Sarwar; Anam Ahmed; Ahmad Shamim; Aanchal Kakkar; A Shariff; Rakesh Kumar; Alok Thakar

doi:10.1007/s12070-023-03623-6

Can Superoxide Anions Predict the Malignant Potential of Carotid body Tumor? - A Pilot Study

Indian J Otolaryngol Head Neck Surg. 2023 Sep;75(3):1819-1825. doi: 10.1007/s12070-023-03623-6. Epub 2023 Apr 8.

Authors

Affiliations

¹ Department of Otorhinolaryngology and Head-Neck Surgery, All India Institute of Medical Sciences (AIIMS), New Delhi Delhi, India.
² Department of Anatomy, AIIMS, New Delhi Delhi, India.
³ Department of Nuclear Medicine, AIIMS, New Delhi Delhi, India.
⁴ Department of Pathology, AIIMS, New Delhi Delhi, India.

Abstract

Currently, there is no consensus on estimating the malignant potential of Carotid Body Tumor (CBT) and the only way to predict a metastatic CBT is through DOTANOC Positron Emission Tomography (PET) scan. There is a well-established correlation between CBT and superoxide anions inside tumor cells. The purpose of this pilot study was to measure superoxide anions inside CBT cells and find if these can be used as marker to predict malignant potential of CBT. The results were also co-related with findings of DOTANOC PET scan retrospectively. The CBT tissue from 10 patients was stained using a fluorogenic dye and superoxide anions were measured by analysis of fluorescent image. The patients were divided into two groups - First group with four patients having potentially malignant CBT based upon clinico-surgical characteristics and second group with the rest of the six patients. It was seen that the superoxide anions were highest in the first group which included patients with metastatic carotid body tumor, patients with multiple paragangliomas and patient with positive family history (p = 0.011). The same patients also had metastasis and multiple tumors detected on DOTANOC PET scan. It was concluded that measuring superoxide anions in excised tumor tissue can be used to estimate malignant potential of CBT and can identify patients who truly require DOTANOC PET scan; without affecting the treatment, as it is an expensive investigation involving ionizing radiation and may not be available in all centres.

Supplementary information: The online version contains supplementary material available at 10.1007/s12070-023-03623-6.

Keywords: Carotid body tumor; Ga-68 DOTANOC PET/CT scan; Oxidative stress; Paraganglioma; Superoxide anion.

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