Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints

Haiming Wang; Chi Zhang; Siyi Zhu; Chengfei Gao; Qiang Gao; Ridong Huang; Sijia Liu; Xiangyang Wei; Huakai Zhang; Quan Wei; Chengqi He

doi:10.1371/journal.pone.0270074

Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints

PLoS One. 2023 Aug 17;18(8):e0270074. doi: 10.1371/journal.pone.0270074. eCollection 2023.

Authors

Haiming Wang^{1

2}, Chi Zhang^{3

4

5}, Siyi Zhu^{6

7}, Chengfei Gao^{8

9}, Qiang Gao^{6

7}, Ridong Huang¹⁰, Sijia Liu^{6

7}, Xiangyang Wei^{1

2}, Huakai Zhang¹¹, Quan Wei^{6

7}, Chengqi He^{6

7}

Affiliations

¹ Rehabilitation Medicine Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
² Center of Rehabilitation Engineering Technology Research, Henan Province, Zhengzhou, Henan, China.
³ Rehabilitation Medicine Department, The Affiliated Hospital Of Southwest Medical University, Luzhou, Sichuan, China.
⁴ Department of Rehabilitation Medicine, Southwest Medical University, Luzhou, Sichuan, China.
⁵ Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou, Sichuan, China.
⁶ Rehabilitation Medical Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
⁷ Rehabilitation Medicine Key Laboratory of Sichuan Province, Sichuan University, Chengdu, Sichuan, China.
⁸ Rehabilitation Medicine Department, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
⁹ Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong, China.
¹⁰ Department of Respiratory and Critical Care Medicine, Targeted Tracer Research and Development Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
¹¹ Medical College of Zhengzhou University of Industrial technology, Zhengzhou, Henan, China.

Abstract

Purposes: To evaluate the effects of low frequency whole-body vibration (WBV) on degeneration of articular cartilage and subchondral bone in mice with destabilization of the medial meniscus (DMM)induced osteoarthritis(OA) and mice with normal knee.

Methods: Ten-week-old C57BL/6J male mice received DMM on right knees, while the left knees performed sham operation. There were six groups: DMM, SHAM DMM, DMM+WBV,SHAM DMM+WBV, DMM+ NON-WBV and SHAM DMM+NON-WBV. After four weeks, the knees were harvested from the DMM and SHAM DMM group. The remaining groups were treated with WBV (10 Hz) or NON-WBV. Four weeks later, the knees were harvested. Genes, containing Aggrecan(Acan) and CollagenⅡ(Col2a1), Matrix Metalloproteinases 3 and 13(MMP3,13), TNFα and IL6, were measured and staining was also performed. OA was graded with OARSI scores, and tibial plateaubone volume to tissue volume ratio(BV/TV), bone surface area to bone volume ratio (BS/BV), trabecular number(Tb.N) and trabecular thickness separation(TS) between groups were analyzed.

Results: Increased OARSI scores and cartilage degradation were observed after WBV. BV/TV, Tb.N and TS were not significant between the groups. Significant reductions were observed in MMP3, MMP13, Col2a1, Acan, TNFα and IL6 in the DMM+WBV compared to SHAM DMM+WBV group. BV/TV, BS/BV, Tb.N, TS and OARSI scores were not significantly changed in the left knees. IL6 expression in the SHAM DMM+WBV group was significantly increased compared with the SHAM DMM+ NON-WBV group, while Col2a1, Acan and MMP13 expression decreased.

Conclusion: WBV accelerated cartilage degeneration and caused slight changes in subchondral bone in a DMM-induced OA model. WBV had no morphologic effect on normal joints.

Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cartilage, Articular*
Interleukin-6 / genetics
Male
Matrix Metalloproteinase 13 / genetics
Matrix Metalloproteinase 3
Mice
Mice, Inbred C57BL
Osteoarthritis, Knee* / therapy
Tumor Necrosis Factor-alpha
Vibration / therapeutic use

Substances

Tumor Necrosis Factor-alpha
Matrix Metalloproteinase 13
Matrix Metalloproteinase 3
Interleukin-6

Grants and funding

The National Natural Science Foundation of China (Grant No. 81572231, 81972146) and the Project of the Science and Technology Department in Sichuan province (Grant No. 2019YJ0119). Sichuan Provincial Department of Science and Technology Fund (2021YJ0463, 2021YFS0070). The Project of the Science and Technology Department in Henan Provincial Department (Grant No. 22170112). The Joint project of Henan Provincial Health Commission (Grant No.LHGJ 20190252).The Natural Science Foundation of China-Henan Province (Grant No. 202300410388). Key Research Projects of Higher Education Institutions in Henan Province(Grant No. 21A320041). Henan Medical Education Research Project (Grant No. Wjlx2020529).