Measuring IL-1β Processing by Bioluminescence Sensors: Using a Bioluminescence Resonance Energy Transfer Biosensor

Vincent Compan; Pablo Pelegrín

doi:10.1007/978-1-0716-3350-2_3

Measuring IL-1β Processing by Bioluminescence Sensors: Using a Bioluminescence Resonance Energy Transfer Biosensor

Methods Mol Biol. 2023:2696:47-53. doi: 10.1007/978-1-0716-3350-2_3.

Authors

Vincent Compan^{1

2}, Pablo Pelegrín^{3

4}

Affiliations

¹ Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France.
² Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France.
³ Department of Bioquimestry and Molecular Biology B and Immunology, Faculty of Medicine, University of Murcia, Murcia, Spain. pablopel@um.es.
⁴ Biomedical Research Institute of Murcia, Murcia, Spain. pablopel@um.es.

PMID: 37578714
DOI: 10.1007/978-1-0716-3350-2_3

Abstract

IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA has been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and in living macrophages either using a plate reader or a microscope.

Keywords: BRET; Bioluminescence; IL-1β; Macrophage; Sensor.