Bacteroides xylanisolvens possesses a potent anti-hyperuricemia effect in goslings fed on a high-protein diet

Ning Song; Mingze Wang; Guangxu Zhong; Kunpeng Zhu; Pengju Chen; Naisheng Zhang; Xiaobo Liu; Wenlong Zhang

doi:10.3389/fmicb.2023.1173856

Bacteroides xylanisolvens possesses a potent anti-hyperuricemia effect in goslings fed on a high-protein diet

Front Microbiol. 2023 Jun 30:14:1173856. doi: 10.3389/fmicb.2023.1173856. eCollection 2023.

Authors

Ning Song^#¹, Mingze Wang^#¹, Guangxu Zhong¹, Kunpeng Zhu¹, Pengju Chen^{2

3}, Naisheng Zhang¹, Xiaobo Liu⁴, Wenlong Zhang¹

Affiliations

¹ Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, China.
² Henan Institute of Modern Chinese Veterinary Medicine, Zhengzhou, Henan, China.
³ Shandong Xindehui Biotechnology Company Ltd., Yuncheng, Shandong, China.
⁴ School of Life Science, Jilin University, Changchun, China.

^# Contributed equally.

Abstract

Introduction: Hyperuricemia is widespread in humans and birds which is a necessary physiological factor leading to gout. Studies have shown an inextricable relationship between gut microbiota and hyperuricemia. This study explored the association between intestinal flora and hyperuricemia in Goslings.

Methods and results: The hyperuricemia model was established in gosling by a high protein diet (HPD). 16S rDNA sequencing showed that the cecal microbiota differed significantly between the HPD and control groups (fed with the normal protein). The abundance of Firmicutes was higher in the HPD group, while the Bacteroidetes were lower than in controls. To investigate the role of intestinal flora in hyperuricemia, the cecum microbiotas from the HPD group and the control group were transplanted to the newly born goslings by gavage. The serum uric acid levels of the goslings that transplanted the cecal microbiota of the HPD group were significantly higher than the goslings that transplanted the cecal microbiota of the controls. Furthermore, the transplantation of cecal microbiota also affects the production and excretion of uric acid in goslings. Then we identify the gut bacterium Bacteroides xylanisolvens as an effective anti-hyperuricemia in the Goslings. B. xylanisolvens reduces serum uric acid concentrations in hyperuricemia in the Goslings' model, and it can up-regulation ABCG2 mRNA expression in the kidney and down-regulation XDH mRNA expression in the liver.

Discussion: The intestinal flora acts as a novel target for the therapeutic approach to hyperuricemia and gout, suggest Bacteroides xylanisolvens is a possible route to therapy for hyperuricemia and gout in goslings.

Keywords: Bacteroides xylanisolvens; goslings; hyperuricemia; intestinal flora; uric acid.

Grants and funding

This work was supported by the National Natural Science Foundation of China (Nos. 32202889 and 31972749), the Natural Science Foundation of Jilin Province (No. 20220101304JC), and the Scientific Research Project of the Education Department of Jilin Province (No. 46005). It was also supported by the Graduate Innovation Fund of Jilin University (2023CX252).