Investigating the etiology of non-carious cervical lesions: Novel µCT analysis

Abstract

Objectives: The etiology of non-carious cervical lesions (NCCLs) is not fully understood, limiting treatment and prevention. Our aim was to evaluate the effect of mechanical loading and acid exposure on the cervical tooth region using a random spectrum loading model that simulates the nature of oral mastication.

Methods: Thirty extracted human premolars were divided into three experimental groups: 1) unloaded teeth immersed in acid (erosion group: Er), 2) loaded teeth immersed in acid (erosion with spectrum loading group: Er-SL), and 3) loaded teeth immersed in distilled water (spectrum loading group: SL). Random spectrum loading with loads ranging from 100 to 500 N was performed. All teeth were scanned using micro-CT. A novel 3D analysis was developed to evaluate the circumferential cervical tissue loss and regions under tension and compression. For parametric and non-parametric comparisons, one-way ANOVA with Tukey post-hoc tests and Kruskal-Wallis with Bonferroni post-hoc tests were used.

Results: A significant difference was observed in the circumferential volumetric loss, with the Er-SL exhibiting the greatest volume loss (p < 0.001). Moreover, in the loaded groups (Er-SL and SL), regions subjected to tension showed significantly greater loss (p < 0.001, p = 0.007) compared with regions subjected to compression.

Conclusions: The novel high-resolution micro-CT analysis provided new insights into the etiology of NCCLs. The results suggested that the cumulative effect of mechanical loading and acid exposure may play a major role in NCCL formation.

Clinical significance: This study investigates the etiology of NCCLs by examining the combined effects of occlusal loads and acid exposure on cervical tissue loss. Understanding the pathogenesis of NCCLs paves the way for the development of improved preventative measures and treatment strategies to prevent tooth structure degradation.

Keywords: Biomechanics; Dentin; Enamel; Tensile strength; Tooth erosion; X-ray micro-computed tomography.