The present paper explains the conditions for semen collection in the dog in principal and the technique of semen collection by manual stimulation of the penis in detail. The evaluation of the most important ejaculate parameters is pointed out concerning their dependence of the partially striking variation of the body size of dogs from different breeds. Furthermore some methods of semen preservation and the results of the laboratory examinations are presented. The notes upon artificial insemination contain the time, frequency, interval and technique of semen application. Better conception rates can be obtained by a double than by a single insemination. Using fresh semen an insemination interval of 48 hours has proved favorable whereas with preserved semen the insemination interval should not exceed 24 hours. While freshly ejaculated semen can be applied with good success intravaginally, the intrauterine insemination offers better conditions for conception using liquid or frozen stored semen.