Parameters optimization of Fe3O4 NPs synthesis by Tamarindus indica leaf extract possessing both peroxidase as well as excellent dye removal activity

Md Rajibul Akanda; Md Al-Amin; Masrifa Akter Mele; Zunayed Mahmud Shuva; Md Billal Hossain; Taohedul Islam; Md Mehedi Hasan; Umme Habiba Ema

doi:10.1016/j.heliyon.2023.e16699

Parameters optimization of Fe₃O₄ NPs synthesis by Tamarindus indica leaf extract possessing both peroxidase as well as excellent dye removal activity

Heliyon. 2023 May 27;9(6):e16699. doi: 10.1016/j.heliyon.2023.e16699. eCollection 2023 Jun.

Authors

Md Rajibul Akanda¹, Md Al-Amin¹, Masrifa Akter Mele¹, Zunayed Mahmud Shuva¹, Md Billal Hossain¹, Taohedul Islam¹, Md Mehedi Hasan¹, Umme Habiba Ema¹

Affiliation

¹ Department of Chemistry, Jagannath University, Dhaka, 1100, Bangladesh.

Abstract

This study reports optimized conditions for the green synthesis of iron (II,III) oxide nanoparticles (Fe₃O₄ NPs) from Tamarindus indica (T. indica) leaf extract. The synthetic parameters like concentration of leaf extract, solvent system, buffer, electrolyte, pH, and time were optimized for Fe₃O₄ NPs synthesis. Fe₃O₄ NPs were obtained from the synthesis protocol by measuring size (80 $\pm$ 3 nm approx.), characteristics color changes, and an absorption peak between 270 nm and 280 nm using a UV-visible spectrophotometer, scanning electron microscope (SEM), and an energy dispersive X-ray spectrometer (EDS) study. Peroxidase activity was tested with 3,3,5,5-Tetramethylbenzidine (TMB) oxidation in the presence of hydrogen peroxide and dye removal activity was tested with malachite green (MG). The results indicated that the successful synthesis of Fe₃O₄ nanoparticles using aqueous leaf extract of T. indica is a practical alternative for biomedical applications due to its potent peroxidase activity and high dye removal capacity (about 93% with UV light and 55% with room light).

Keywords: Dye removal activity; Fe3O4 nanoparticle; Green synthesis; Peroxidase activity; Tamarindus indica leaf Extract.