13C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices

Elisa Motori; Patrick Giavalisco

doi:10.1007/978-1-0716-3247-5_14

¹³C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices

Methods Mol Biol. 2023:2675:181-194. doi: 10.1007/978-1-0716-3247-5_14.

Authors

Elisa Motori^{1

2}, Patrick Giavalisco³

Affiliations

¹ Institute of Biochemistry, University of Cologne, Cologne, Germany. elisa.motori@uni-koeln.de.
² Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany. elisa.motori@uni-koeln.de.
³ Max Planck Institute for Biology of Ageing, Cologne, Germany.

PMID: 37258764
DOI: 10.1007/978-1-0716-3247-5_14

Abstract

Feeding of stable ¹³C-labeled compounds coupled to mass spectrometric analysis has enabled the characterization of dynamic metabolite partitioning in various experimental conditions. This information is particularly relevant for the study and functional understanding of brain metabolic heterogeneity. We here describe a protocol for the analysis of metabolic enrichment analysis upon feeding of murine acute cerebellar slices with ¹³C-labeled substrates.

Keywords: 13C labeling; Brain slices; Cellular metabolism; Cerebellum; Isotope enrichment; Liquid chromatography; Mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain*
Carbon Isotopes / chemistry
Isotope Labeling / methods
Mass Spectrometry
Mice

Substances

Carbon Isotopes