Dual recognition elements for selective determination of progesterone based on molecularly imprinted electrochemical aptasensor

Yan Huang; Daixin Ye; Jing Yang; Wenyi Zhu; Li Li; Yaping Ding

doi:10.1016/j.aca.2023.341288

Dual recognition elements for selective determination of progesterone based on molecularly imprinted electrochemical aptasensor

Anal Chim Acta. 2023 Jul 11:1264:341288. doi: 10.1016/j.aca.2023.341288. Epub 2023 Apr 26.

Authors

Yan Huang¹, Daixin Ye², Jing Yang¹, Wenyi Zhu³, Li Li⁴, Yaping Ding⁵

Affiliations

¹ Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China.
² College of Sciences & Institute for Sustainable Energy, Shanghai University, Shanghai, 200444, PR China.
³ Shanghai University Hospital, Shanghai University, Shanghai, 200444, PR China.
⁴ Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address: lilidu@shu.edu.cn.
⁵ Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China; Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University, Shanghai, 200444, PR China. Electronic address: wdingyp@sina.com.

PMID: 37230721
DOI: 10.1016/j.aca.2023.341288

Abstract

A novel molecularly imprinted electrochemical aptasensor (MIEAS) was constructed for selective progesterone (P4) detection based on SnO₂-graphene (SnO₂-Gr) nanomaterial and gold nanoparticles (AuNPs). SnO₂-Gr with a large specific area and excellent conductivity improved the adsorption capacity of P4. Aptamer, as biocompatible monomer, was captured by AuNPs on modified electrode through Au-S bond. An electropolymerized molecularly imprinted polymer (MIP) film consisted of p-aminothiophenol as chemical functional monomer and P4 as template molecule. Due to the synergetic effect of MIP and aptamer towards P4, this MIEAS exhibited better selectivity than the sensor with MIP or aptamer as single recognition element. The prepared sensor had a low detection limit of 1.73 × 10^-15 M in a wide linear range from 10^-14 M to 10^-5 M. Satisfactory recovery obtained in tap water and milk samples proved that this sensor had great potential in environmental and food analysis.

Keywords: Aptamer; Dual recognition elements; Electrochemical sensor; Molecularly imprinted polymer; Progesterone.

MeSH terms

Electrochemical Techniques
Electrodes
Gold / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry
Molecular Imprinting*
Molecularly Imprinted Polymers
Polymers / chemistry
Progesterone

Substances

Polymers
Gold
Progesterone
Molecularly Imprinted Polymers