Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos

Abstract

CUT&Tag is a method to map the genome-wide distribution of histone modifications and some chromatin-associated proteins. CUT&Tag relies on antibody-targeted chromatin tagmentation and can easily be scaled up or automatized. This protocol provides clear experimental guidelines and helpful considerations when planning and executing CUT&Tag experiments.

Keywords: CUT&Tag (Cleavage Under Targets and Tagmentation); Drosophila embryos; Histone modifications.