Single-cell immune profiling of Meniere Disease patients

Clin Immunol. 2023 Jul:252:109632. doi: 10.1016/j.clim.2023.109632. Epub 2023 May 11.

Abstract

Background: Meniere Disease (MD) is an inner ear syndrome, characterized by episodes of vertigo, tinnitus and fluctuating sensorineural hearing loss. The pathological mechanism leading to sporadic MD is still poorly understood, however an allergic inflammatory response seems to be involved in some patients with MD.

Objective: Decipher an immune signature associated with the syndrome.

Methods: We performed mass cytometry immune profiling on peripheral blood from MD patients and controls. We analyzed differences in state and differences in abundance of the different cellular subsets. IgE levels were quantified through ELISA on supernatant of cultured whole blood.

Results: We have identified two clusters of individuals according to the single cell cytokine profile. These clusters presented differences in IgE levels, immune cell population abundance, including a reduction of CD56dim NK-cells, and changes in cytokine expression with a different response to bacterial and fungal antigens.

Conclusion: Our results support a systemic inflammatory response in some MD patients that show a type 2 response with allergic phenotype, which could benefit from personalized IL-4 blockers.

Keywords: Allergy; Cytokines; Inflammation; Mass cytometry; Meniere Disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytokines
  • Hearing Loss, Sensorineural* / complications
  • Humans
  • Immunoglobulin E
  • Meniere Disease* / complications
  • Meniere Disease* / epidemiology
  • Syndrome
  • Vertigo / complications

Substances

  • Cytokines
  • Immunoglobulin E