Ecological insight into deterioration of one-stage partial nitritation and anammox system during environmental disturbance

Abstract

This study investigated the relationship between the performance and the ecological features of a one-stage partial nitritation and anammox disturbed by oxygen. The disturbance caused an irreversible deterioration of the nitrogen removal rate from 0.8 to 0.05 kg N/(m³∙d) although the anammox genera increased from 1% to 1.4%. Meanwhile, the richness and evenness reduced from 455 and 4.00 to 429 and 3.81, respectively, following a similar pattern to the community complexity. The community drifted and formed three distinct clusters during and after the disturbance. Furthermore, 234 of 634 operational taxonomic units in the community were depleted despite recovered diversity and complexity during long-term stable operation. In conclusion, the ecological fluctuation of the microbial community with decreasing resilience was the driving force that fatally collapsed the system performance. This study suggests that ecological features are conducive to the diagnosis, prediction, and optimization of a partial nitritation and anammox system.

Keywords: Autotrophic nitrogen removal; Diversity; Microbial community; Successional pattern.