Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles

Yang Li; Yunpeng Wang; Jinwei Tian; Jian-An Huang

doi:10.1007/978-1-0716-3203-1_2

Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles

Methods Mol Biol. 2023:2668:15-22. doi: 10.1007/978-1-0716-3203-1_2.

Authors

Yang Li¹, Yunpeng Wang¹, Jinwei Tian², Jian-An Huang³

Affiliations

¹ College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China.
² Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, The Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, Heilongjiang, China.
³ Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland. jianan.huang@oulu.fi.

PMID: 37140786
DOI: 10.1007/978-1-0716-3203-1_2

Abstract

Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.

Keywords: Cell sample; Exosome; Label-free; Raman scattering; SERS; Silver nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Exosomes* / metabolism
Metal Nanoparticles*
Proteins / metabolism
Silver
Spectrum Analysis, Raman / methods

Substances

Silver
Proteins