Frequency of the rs2015 (T>G) and rs2241703 (G>A) polymorphisms in the miRNA-SIRT2 gene in type 2 diabetes mellitus in Saudi Arabia

Saudi Med J. 2023 Apr;44(4):363-367. doi: 10.15537/smj.2023.44.4.20220863.

Abstract

Objectives: To investigate the prevalence of rs2015 (T>G) and rs2241703 (G>A) polymorphisms in the miRNA-SIRT2 gene in Saudi Arabia and their possible associations with type 2 diabetes mellitus (T2DM).

Methods: Blood samples were collected from 428 participants from Jazan University Hospital, Jazan, Saudi Arabia between September 2021 and June 2022 and subjected to TaqMan single-nucleotide polymorphisms (SNP) genotyping assay for rs241703 (G>A) and rs2015 (G>T). Genotype frequencies were determined in control (n=217).

Results: The A allele of rs2241703 was undetected in our population, and all samples carried the GG genotype. The rs2015 SNP frequency was 29.4% for GG, 45.6% for GT, and 24% for TT. However, logistic regression analysis of the dominant inheritance model showed no association between the T allele and T2DM calculated odds ratio [OR]=0.80, 95% confidence interval=0.53 to 1.20, p=0.301).

Conclusion: Although rs2241703 SNP of Sirtuins 2 is not present, rs2015 SNP is highly prevalent in Saudi Arabia, but no direct link was identified with T2DM.

Keywords: SIRT2; Saudi Arabia; T2DM; polymorphism.

MeSH terms

  • Case-Control Studies
  • Diabetes Mellitus, Type 2* / epidemiology
  • Diabetes Mellitus, Type 2* / genetics
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • MicroRNAs* / genetics
  • Polymorphism, Single Nucleotide
  • Saudi Arabia / epidemiology
  • Sirtuin 2 / genetics

Substances

  • MicroRNAs
  • Sirtuin 2
  • SIRT2 protein, human