Developmentally programmed histone H3 expression regulates cellular plasticity at the parental-to-early embryo transition

Sci Adv. 2023 Apr 7;9(14):eadh0411. doi: 10.1126/sciadv.adh0411. Epub 2023 Apr 7.

Abstract

During metazoan development, the marked change in developmental potential from the parental germline to the embryo raises an important question regarding how the next life cycle is reset. As the basic unit of chromatin, histones are essential for regulating chromatin structure and function and, accordingly, transcription. However, the genome-wide dynamics of the canonical, replication-coupled (RC) histones during gametogenesis and embryogenesis remain unknown. In this study, we use CRISPR-Cas9-mediated gene editing in Caenorhabditis elegans to investigate the expression pattern and role of individual RC histone H3 genes and compare them to the histone variant, H3.3. We report a tightly regulated epigenome landscape change from the germline to embryos that are regulated through differential expression of distinct histone gene clusters. Together, this study reveals that a change from a H3.3- to H3-enriched epigenome during embryogenesis restricts developmental plasticity and uncovers distinct roles for individual H3 genes in regulating germline chromatin.

MeSH terms

  • Animals
  • Caenorhabditis elegans / metabolism
  • Cell Plasticity*
  • Chromatin / genetics
  • Embryo, Mammalian / metabolism
  • Histones* / genetics
  • Histones* / metabolism

Substances

  • Histones
  • Chromatin