Reduction of peritoneal cavity B1a cells in adult Slc7a5 knockdown mice via dysregulating the mTOR pathway

Yumeng Sun; Junjie Wen; Tao Xu; Lu Meng

doi:10.1016/j.intimp.2023.109939

Reduction of peritoneal cavity B1a cells in adult Slc7a5 knockdown mice via dysregulating the mTOR pathway

Int Immunopharmacol. 2023 Apr:117:109939. doi: 10.1016/j.intimp.2023.109939. Epub 2023 Mar 10.

Authors

Yumeng Sun¹, Junjie Wen¹, Tao Xu², Lu Meng³

Affiliations

¹ CAS Key Laboratory of Molecular Virology and Immunology, Chinese Academy of Sciences, Shanghai, China University of Chinese Academy of Sciences, Beijing, China.
² Department of Infectious Diseases, National Medical Center for Infectious Diseases, Huashan Hospital, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
³ CAS Key Laboratory of Molecular Virology and Immunology, Chinese Academy of Sciences, Shanghai, China University of Chinese Academy of Sciences, Beijing, China. Electronic address: lmeng@ips.ac.cn.

PMID: 37012862
DOI: 10.1016/j.intimp.2023.109939

Abstract

Slc7a5 is an important amino acid transporter that is highly expressed in metabolically active and rapidly proliferating cells. To explore the effect of Slc7a5 on adult B cell development, we conditionally deleted Slc7a5 in murine B cells and observed a significant reduction of B1a cells. In contrast to PI3K-Akt pathway activation, activity of the mTOR pathway was decreased. This may result from intracellular amino acid starvation in Slc7a5 knockdown (Slc7a5 KD) bone marrow B cells, thereby dampening B1a development. RNA-seq analysis demonstrated increased translation and reduced proliferation in Slc7a5 KD bone marrow B cells. Overall, the results of our study highlight the importance of Slc7a5 in peritoneal B1a cell development.

Keywords: B1a cell; Peritoneal cavity; Slc7a5; mTOR pathway.

MeSH terms

Animals
B-Lymphocytes* / metabolism
Cell Proliferation
Large Neutral Amino Acid-Transporter 1* / genetics
Large Neutral Amino Acid-Transporter 1* / metabolism
Mice
Peritoneal Cavity*
Phosphatidylinositol 3-Kinases / metabolism
TOR Serine-Threonine Kinases / metabolism

Substances

Large Neutral Amino Acid-Transporter 1
Phosphatidylinositol 3-Kinases
TOR Serine-Threonine Kinases
Slc7a5 protein, mouse