Grass-Shaped Zinc Oxide Nanoparticles Synthesized by the Sol-Gel Process and Their Antagonistic Properties towards the Biotrophic Parasite, Meloidogyne incognita

Amir Khan; Azmat Ali Khan; Mohd Jameel; Mohd Farhan Khan; Masudulla Khan; Arshad Khan; Faheem Ahmad; Mahboob Alam

doi:10.1155/2023/6834710

Grass-Shaped Zinc Oxide Nanoparticles Synthesized by the Sol-Gel Process and Their Antagonistic Properties towards the Biotrophic Parasite, Meloidogyne incognita

Bioinorg Chem Appl. 2023 Mar 24:2023:6834710. doi: 10.1155/2023/6834710. eCollection 2023.

Authors

Amir Khan¹, Azmat Ali Khan², Mohd Jameel³, Mohd Farhan Khan⁴, Masudulla Khan⁵, Arshad Khan¹, Faheem Ahmad¹, Mahboob Alam⁶

Affiliations

¹ Department of Botany, Aligarh Muslim University, Aligarh 202002, India.
² Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
³ Department of Zoology, Aligarh Muslim University, Aligarh 202002, India.
⁴ Department of Science, Gagan College of Management and Technology, Aligarh 202001, India.
⁵ Botany Section, Women's College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
⁶ Department of Safety Engineering, Dongguk University, 123 Dongdae-ro, Gyeongju-si, Gyeongbuk 780714, Republic of Korea.

Abstract

The presence of Meloidogyne spp., also known as root-knot nematodes, presents a significant danger to global agricultural progress. Since chemical nematicides have high levels of toxicity, it is imperative to develop environmentally friendly methods to manage root-knot nematodes. Nanotechnology is now the most progressive way to attract researchers due to its innovative quality in combating plant diseases. Our study focused on the sol-gel process to synthesize grass-shaped zinc oxide nanoparticles (G-ZnO NPs) and assess its nematicidal behavior against Meloidogyne incognita. Various concentrations (250, 500, 750, and 1000 ppm) of G-ZnO NPs were utilized to expose both the infectious stage (J2s) and egg masses of M. incognita. Laboratory results revealed that G-ZnO NPs showed toxicity to J2s with LC50 values of 1352.96, 969.64, and 621.53 ppm at 12, 24, and 36 hours, respectively, and the result was the inhibition of egg hatching in M. incognita. All three exposure periods were reported linked with the concentration strength of G-ZnO NPs. The pot experiment results exhibited that G-ZnO NPs significantly reduced the root-gall infection of chickpea plants under M. incognita attack. Compared with the untreated control, there was a significant improvement in plant growth attributes and physiological parameters as well, when distinct G-ZnO NP doses (250, 500, 750, and 1000 ppm) were applied. In the pot study, we noticed a reduction in the root-gall index with an increase in the concentration of G-ZnO NPs. The results confirmed that G-ZnO NPs have enormous potential in sustainable agriculture for controlling the root-knot nematode, M. incognita, in chickpea production.