Screening of a short chain antimicrobial peptide-LKLHI and its application in hydrogels for wound healing

Abstract

Antibacterial peptides have been widely used in the field of antibacterial due to their biocompatibility. In this work, owing to quickly screen out peptides with antibacterial effects, the bacterial membranes of E. coli and S. aureus were extracted and fixed on self-made silica gel microspheres to prepare bacterial membrane chromatography stationary phase. We successfully screened antimicrobial peptides from a peptide library composed of one-bead-one-compound by bacterial membrane chromatography. The antibacterial peptide has an effective defense effect on gram-positive bacteria, gram-negative bacteria, and fungi. In addition, the antibacterial peptide has almost no hemolysis and cytotoxicity and other excellent biocompatibility and has excellent properties such as stability, broad-spectrum antibacterial, and promotion of wound healing，and HA hydrogel carrier loaded with antimicrobial peptides was prepared, which provided the application direction of antimicrobial dressings for antimicrobial peptides. In summary, this method can screen out polypeptides with antibacterial effects, and the screened-out antibacterial peptides are expected to be applied in clinical applications.

Keywords: Antimicrobial peptides; Bacterial membrane chromatography; Hydrogel; Screening; Wound healing.