Adenosine analogs with covalently attached lipids have enhanced potency at A1-adenosine receptors

FEBS Lett. 1987 Dec 10;225(1-2):97-102. doi: 10.1016/0014-5793(87)81138-9.

Abstract

Chemically functionalized congeners of N6-phenyladenosine and 1,3-dipropyl-8-phenylxanthine have been covalently coupled to fatty acids, diglycerides, and a phospholipid. The lipid-drug conjugates inhibit R-[3H]-phenylisopropyladenosine binding to A1-adenosine receptors in rat cerebral cortex membranes. A xanthine-phosphatidylethanolamine conjugate bound with a Ki value of 19 nM. Various xanthine esters of low potency are potential prodrugs. Amides of an adenosine amine congener (ADAC) with 18-carbon fatty acids exhibited Ki values at A1-adenosine receptors of 70 pM, representing a 130-fold enhancement over the affinity of the corresponding acetyl amide. The very high affinity of adenosine-lipid conjugates may be due to stabilization of these adducts in the phospholipid microenvironment of the receptor protein.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / metabolism
  • Aniline Compounds / metabolism
  • Animals
  • Cell Membrane / metabolism
  • Cerebral Cortex / metabolism
  • Chemical Phenomena
  • Chemistry
  • Diglycerides / metabolism
  • Fatty Acids / metabolism
  • Lipid Metabolism*
  • Phenylisopropyladenosine / metabolism
  • Phosphatidylethanolamines / metabolism
  • Prodrugs / metabolism
  • Rats
  • Receptors, Purinergic / metabolism*
  • Xanthines / metabolism

Substances

  • Aniline Compounds
  • Diglycerides
  • Fatty Acids
  • Phosphatidylethanolamines
  • Prodrugs
  • Receptors, Purinergic
  • Xanthines
  • N(6)-phenyladenosine
  • Phenylisopropyladenosine
  • 1,3-dipropyl-8-phenylxanthine
  • Adenosine