LRRK2 Quantification in Cerebrospinal Fluid of Patients with Parkinson's Disease and Atypical Parkinsonian Syndromes

Mov Disord. 2023 Apr;38(4):682-688. doi: 10.1002/mds.29336. Epub 2023 Feb 18.

Abstract

Background: The alteration of leucine-rich repeat kinase 2 (LRRK2) kinase activity is thought to be involved in Parkinson's disease (PD) pathogenesis beyond familiar cases, and LRRK2 inhibitors are currently under investigation. Preliminary data suggest a relationship between LRRK2 alteration and cognitive impairment in PD.

Objective: To investigate cerebrospinal fluid (CSF) LRRK2 levels in PD and other parkinsonian disorders, also correlating them with cognitive impairment.

Methods: In this study, we retrospectively investigated by means of a novel highly sensitive immunoassay the levels of total and phosphorylated (pS1292) LRRK2 in CSF of cognitively unimpaired PD (n = 55), PD with mild cognitive impairment (n = 49), PD with dementia (n = 18), dementia with Lewy bodies (n = 12), atypical parkinsonian syndromes (n = 35), and neurological controls (n = 30).

Results: Total and pS1292 LRRK2 levels were significantly higher in PD with dementia with respect to PD with mild cognitive impairment and PD, and also showed a correlation with cognitive performances.

Conclusions: The tested immunoassay may represent a reliable method for assessing CSF LRRK2 levels. The results appear to confirm an association of LRRK2 alteration with cognitive impairment in PD. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.

Keywords: LRRK2; Parkinson's disease; atypical parkinsonian syndromes; cognitive impairment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dementia* / etiology
  • Dementia* / metabolism
  • Humans
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2* / chemistry
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2* / metabolism
  • Mutation
  • Parkinson Disease* / cerebrospinal fluid
  • Parkinson Disease* / complications
  • Parkinsonian Disorders* / cerebrospinal fluid
  • Parkinsonian Disorders* / complications
  • Retrospective Studies

Substances

  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
  • LRRK2 protein, human