Two colors, one-step, self-drive fluorescent strategy for chloramphenicol detection base on DNAzyme cleavage triggered hybridization chain reaction

Abstract

A two colors, one-step, self-drive fluorescent strategy was developed for chloramphenicol (CAP) detection based on cyclic cleavage of molecular beacon (MB) by pincer DNA sequences. CAP can bind with its aptamer and active the enzyme-strand (E-DNA). Then the E-DNA can circularly cleave the MB on the both side of pincer DNA sequences. The cleaved fragments can self-assembly to form a long duplex and cause the great recovery of the two colors fluorescent signal. The limit of detection was as low as 0.7 pM. Importantly, the whole detection process is very simple with only one-step operation. Moreover, the two colors fluorescent signals can greatly enhance the accuracy of the result. It was also successfully used to detect CAP in actual samples.

Keywords: Chloramphenicol; One-step; Pincer DNA sequences; Two colors.