CRISPR-Cas has a new juggling act: interplay between nuclease and protease

Anthony D Rish; Tian-Min Fu

doi:10.1038/s41594-022-00917-1

CRISPR-Cas has a new juggling act: interplay between nuclease and protease

Nat Struct Mol Biol. 2023 Feb;30(2):126-128. doi: 10.1038/s41594-022-00917-1.

Authors

Anthony D Rish^{1

2

3}, Tian-Min Fu^{4

5

6}

Affiliations

¹ Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA.
² The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA.
³ Program of OSBP, The Ohio State University, Columbus, OH, USA.
⁴ Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA. fu.978@osu.edu.
⁵ The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA. fu.978@osu.edu.
⁶ Program of OSBP, The Ohio State University, Columbus, OH, USA. fu.978@osu.edu.

Abstract

Craspase is a newly identified Type III CRISPR-Cas system with two major components: Cas7-11 with nuclease activity and TPR-CHAT with protease activity. Craspases perform a delicate balancing act between nuclease and protease activity to achieve immune tolerance and immune defense in bacteria. See Ekundayo et al.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

CRISPR-Associated Proteins* / metabolism
CRISPR-Cas Systems* / genetics
Endopeptidases
Gene Editing
Peptide Hydrolases

Substances

Peptide Hydrolases
Endopeptidases
CRISPR-Associated Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding