Analysis of Physiological Control of Adult Drosophila Oogenesis by Interorgan Communication

Methods Mol Biol. 2023:2626:89-107. doi: 10.1007/978-1-0716-2970-3_5.

Abstract

Tissue homeostasis is dependent on the interaction between various organs within an organism in response to physiological inputs. The adult Drosophila melanogaster ovary is sensitive to environmental challenges and has recently been shown to be regulated by signaling from peripheral organs. To dissect the intricate coordination between overall organism health and reproduction, it is necessary to meticulously characterize both experimental tools and oogenesis processes. This chapter provides a guide for the careful analysis of interorgan communication in regulating oogenesis in adult Drosophila melanogaster.

Keywords: Drosophila; Follicle cell; Follicle stem cell; Gal4/UAS; Germline cyst; Germline stem cell; Oogenesis; Physiology.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Drosophila Proteins* / genetics
  • Drosophila melanogaster
  • Drosophila*
  • Female
  • Oogenesis / physiology
  • Ovary / physiology

Substances

  • Drosophila Proteins