Photoelectrochemical immunosensor for HER2 detection based on BiVO4-Bi2S3 heterojunction as photoactive material and CdS as signal probe

Qin Zeng; Suiping Wang; Yong Qian; Minghui Yang; Limin Lu

doi:10.1007/s00604-022-05628-4

Photoelectrochemical immunosensor for HER2 detection based on BiVO₄-Bi₂S₃ heterojunction as photoactive material and CdS as signal probe

Mikrochim Acta. 2023 Jan 24;190(2):67. doi: 10.1007/s00604-022-05628-4.

Authors

Qin Zeng^{1

2}, Suiping Wang³, Yong Qian⁴, Minghui Yang⁵, Limin Lu⁶

Affiliations

¹ Institute of Functional Materials and Agricultural Applied Chemistry, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang, 330045, China.
² Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
³ Hunan Provincial Collaborative Innovation Center for Efficient and Health Production of Fisheries, Hunan University of Arts and Science, Hunan Changde, 415000, China.
⁴ Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang, 330013, China. yqian@ecut.edu.cn.
⁵ Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China. yangminghui@csu.edu.cn.
⁶ Institute of Functional Materials and Agricultural Applied Chemistry, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang, 330045, China. lulimin816@hotmail.com.

PMID: 36692640
DOI: 10.1007/s00604-022-05628-4

Abstract

A sandwiched photoelectrochemical (PEC) sensor was developed for sensitive detection of human epidermal growth factor receptor 2 (HER2) based on BiVO₄-Bi₂S₃ heterojunction as the photoelectric material accompanied with magnetic nanoparticles for enrichment of HER2 and CdS for signal amplification. The in situ generation of Bi₂S₃ on the surface of BiVO₄ forming a BiVO₄-Bi₂S₃ heterojunction is more conducive to the transit of electron-hole pairs. Antibody-modified MNs are utilized to capture and separate HER2 from samples. After forming a sandwich immune structure, under illumination, the photocurrent shows an increasing trend with the increment of HER2 concentration. The PEC immunosensor displays a good linear concentration range between 1.00 and 1.00 × 10³ pg·mL^-1 and a low limit of detection down to 0.680 pg·mL^-1 (S/N = 3) for HER2 under a bias voltage of 0.1 V (vs. Ag/AgCl electrode). Furthermore, the sensor was successfully applied to detect HER2 in serum samples with recoveries that ranged between 96.1 and 114% with RSDs between 1.3 and 5.9%.

Keywords: BiVO4-Bi2S3 heterojunction; CdS; Human epidermal growth factor receptor 2; Magnetic nanoparticles; Photoelectrochemical immunosensor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Humans
Immunoassay
Limit of Detection
Nanoparticles*

Abstract

Publication types

MeSH terms

Grants and funding