A Promising Biomolecule Able to Degrade Neutrophil Extracellular Traps: Cdc PDE, a Rattlesnake Phosphodiesterase

Isadora Oliveira; Victor Costa; Flávio Veras; Isabela Ferreira; Fernando Cunha; Thiago Cunha; Wuelton Monteiro; Eliane Arantes; Manuela Pucca

doi:10.3390/toxins15010044

A Promising Biomolecule Able to Degrade Neutrophil Extracellular Traps: Cdc PDE, a Rattlesnake Phosphodiesterase

Toxins (Basel). 2023 Jan 5;15(1):44. doi: 10.3390/toxins15010044.

Authors

Affiliations

¹ Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, SP, Brazil.
² Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14049-900, SP, Brazil.
³ School of Health Sciences, Amazonas State University, Manaus 69850-000, AM, Brazil.
⁴ Department of Teaching and Research, Dr. Heitor Vieira Dourado Tropical Medicine Foundation, Manaus 69850-000, AM, Brazil.
⁵ Medical School, Federal University of Roraima, Boa Vista 69310-000, RR, Brazil.
⁶ Health Sciences Postgraduate Program, Federal University of Roraima, Boa Vista 69310-000, RR, Brazil.

Abstract

Neutrophil extracellular traps (NETs) are an important mechanism for defense against pathogens. Their overproduction can be harmful since excessive NET formation promotes inflammation and tissue damage in several diseases. Nucleases are capable to degrade NET on basis of their DNA hydrolysis activity, including the CdcPDE, a nuclease isolated from Crotalus durissus collilineatus snake venom. Here, we report a new finding about CdcPDE activity, demonstrating its efficiency in degrading cell-free DNA from NETs, being a potential candidate to assist in therapies targeting inflammatory diseases.

Keywords: CdcPDE; phosphodiesterase; snake venom; svPDE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Crotalus / genetics
Extracellular Traps* / metabolism
Hydrolysis
Neutrophils
Phosphoric Diester Hydrolases / metabolism
Snake Venoms / metabolism

Substances

Phosphoric Diester Hydrolases
Snake Venoms

Grants and funding

This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, São Paulo Research Foundation, scholarship to I.O. 2020/13176-3, and grants 2019/10173-6, 2013/08216-2), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Coordination for the Improvement of Higher Education Personnel, Finance Code 001, scholarship to I.F., and grant 88887.513530/2020-00), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, The National Council for Scientific and Technological Development, scholarship to M.P. No. 307184/2020-0, W.M. No. 309207/2020-7, and E.A. 309399/2021-1). W.M. acknowledges funding support from Fundação de Amparo à Pesquisa do Estado do Amazonas (PAPAC 005/2019, PRO-ESTADO and POSGRAD calls).