PARP1 Gene Knockout Suppresses Expression of DNA Base Excision Repair Genes

Dokl Biochem Biophys. 2023 Feb;508(1):6-11. doi: 10.1134/S1607672922700028. Epub 2023 Jan 18.

Abstract

The effect of PARP1 knockout in HEK293 cells on the gene expression of DNA base excision repair (BER) proteins was studied. It was shown that the expression of all differentially expressed genes (DEGs) of BER was reduced by knockout. The expression of the DNA glycosylase gene NEIL1, which is considered to be one of the common "hubs" for binding BER proteins, has changed the most. The expression of genes of auxiliary subunits of DNA polymerases δ and ε is also significantly reduced. The PARP1 gene knockout cell line obtained is an adequate cell model for studying the activity of the BER process in the absence of PARP1 and testing drugs aimed at inhibiting repair processes. It has been found for the first time that knockout of the PARP1 gene results in a significant change in the level of expression of proteins responsible for ribosome biogenesis and the functioning of the proteasome.

Keywords: DEG; DNA Base Excision Repair; PARP1; PARP1 knockout.

MeSH terms

  • DNA
  • DNA Glycosylases* / genetics
  • DNA Glycosylases* / metabolism
  • DNA Repair
  • Gene Knockout Techniques
  • HEK293 Cells
  • Humans
  • Poly (ADP-Ribose) Polymerase-1 / genetics
  • Poly (ADP-Ribose) Polymerase-1 / metabolism
  • Poly(ADP-ribose) Polymerases* / genetics

Substances

  • Poly(ADP-ribose) Polymerases
  • DNA
  • NEIL1 protein, human
  • DNA Glycosylases
  • PARP1 protein, human
  • Poly (ADP-Ribose) Polymerase-1