Clinical pathology of primary central nervous system lymphoma in HIV-positive patients-a 41 Chinese patients retrospective study

Jiamin Chen; Lei Sun; Yuyang Dai; Liang Zhang; Kun Yang; Xiaoyi Han; Xinghuan Ding; Haili Gao; Xingang Zhou; Peng Wang

doi:10.1016/j.anndiagpath.2023.152108

Clinical pathology of primary central nervous system lymphoma in HIV-positive patients-a 41 Chinese patients retrospective study

Ann Diagn Pathol. 2023 Apr:63:152108. doi: 10.1016/j.anndiagpath.2023.152108. Epub 2023 Jan 7.

Authors

Jiamin Chen¹, Lei Sun¹, Yuyang Dai², Liang Zhang¹, Kun Yang¹, Xiaoyi Han¹, Xinghuan Ding³, Haili Gao¹, Xingang Zhou¹, Peng Wang⁴

Affiliations

¹ Department of Pathology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
² National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
³ Department of Neurosurgery, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
⁴ Department of Pathology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. Electronic address: chinaroc001@126.com.

PMID: 36638601
DOI: 10.1016/j.anndiagpath.2023.152108

Abstract

Objectives: The purpose of this study was to investigate the clinicopathological characteristics of primary central nervous system lymphoma (PCNSL).

Methods: We collected 41 PCNSL formalin-fixed, paraffin-embedded (FFPE) samples from human immunodeficiency virus (HIV)-positive patients and performed HE (haematoxylin-eosin) staining, immunohistochemistry (IHC) staining, in situ hybridization, fluorescence in situ hybridization (FISH). Real-time quantitative polymerase chain reaction (RT-qPCR) was performed in 9 cases of FFPE samples. Meanwhile, we analysed the clinical pathological significance of the results.

Results: Seven patients had diffuse large B-cell lymphoma (DLBCL) with germinal centre B-cell (GCB)-like DLBCL, 32 had activated B-cell (ABC)-like DLBCL, and 2 had Burkitt lymphoma (BL). GCB-like DLBCL patients were older at onset (P = 0.040).A lower CD4⁺ T-cell count and a decrease in cerebrospinal fluid (CSF) glucose content were more frequent in ABC-like DLBCL (P = 0.012, P = 0.006). Overexpression of P53 was more in ABC-like DLBCL (P = 0.041). 73.2 % cases were Epstein-Barr encoding region (EBER) positive, which was more likely in ABC-like DLBCL patients (P = 0.037). EBV DNA were detected in 5/7 EBER-negative DLBCL cases and none (0/2) of the BL cases. All the cases were negative for HHV8 staining. None of the 7 Double expressor lymphoma (DEL) cases had BCL2, BCL6, or c-MYC genetic rearrangements.

Conclusions: HIV-related PCNSL showed unique clinical pathological significance. None of EBV detected in HIV-related BL and without HHV8 infectious are new sights in our single-center study of Chinese HIV-related PCNSL patients.

Keywords: Clinical pathology; HIV; Primary central nervous system lymphoma (PCNSL).

MeSH terms

Central Nervous System / pathology
East Asian People
HIV Infections* / complications
Humans
In Situ Hybridization, Fluorescence
Lymphoma, Large B-Cell, Diffuse* / pathology
Retrospective Studies