In utero intracerebroventricular delivery of adeno-associated viral vectors to target mouse choroid plexus and cerebrospinal fluid

Ahram Jang; Maria K Lehtinen

doi:10.1016/j.xpro.2022.101975

In utero intracerebroventricular delivery of adeno-associated viral vectors to target mouse choroid plexus and cerebrospinal fluid

STAR Protoc. 2023 Mar 17;4(1):101975. doi: 10.1016/j.xpro.2022.101975. Epub 2022 Dec 28.

Authors

Ahram Jang¹, Maria K Lehtinen²

Affiliations

¹ Department of Pathology, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: ahram.jang@childrens.harvard.edu.
² Department of Pathology, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: maria.lehtinen@childrens.harvard.edu.

Abstract

Experimentally targeting mouse choroid plexus (ChP) provides a valuable approach for investigating mechanisms of ChP-cerebrospinal fluid (CSF) biology. Here, we provide a protocol to deliver adeno-associated viral vectors (AAVs) by in utero intracerebroventricular (ICV) injection to ChP epithelial cells. We begin by describing steps for induction anesthesia of the pregnant dam, laparotomy, and in utero ICV injection. We also detail post-surgical care and immunoblot validation. For complete details on the use and execution of this protocol, please refer to Jang et al. (2022).¹.

Keywords: Developmental biology; Gene Expression; Model Organisms; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Choroid Plexus*
Epithelial Cells*
Female
Mice
Pregnancy

Abstract

Publication types

MeSH terms

Grants and funding