Concentration dependence of the binding constant of antibodies

Mol Immunol. 1987 Jul;24(7):715-7. doi: 10.1016/0161-5890(87)90053-8.

Abstract

By simultaneous dilution of the antigen (AG) and the antibody (AB) used in an immune precipitation reaction, at the equivalence AG/AB ratio, it was found that the association constant, Ka, increased from 1.7 X 10(7) 1/M at the highest concn of AG and AB, to 6.5 X 10(11) 1/M at 100-fold dilution of both. An important (but not the only) reason for this increase is taken to be the fact that the AB solution was polyclonal.

MeSH terms

  • Antibody Affinity*
  • Antigen-Antibody Reactions*
  • Dose-Response Relationship, Immunologic
  • Immunoglobulin G / immunology
  • Serum Albumin, Bovine / immunology

Substances

  • Immunoglobulin G
  • Serum Albumin, Bovine