Synthesis, radiolabeling, and preclinical in vivo evaluation of 68Ga-radiolabelled nanodiamonds

Thomas Wanek; Severin Mairinger; Marco Raabe; Md Noor A Alam; Thomas Filip; Johann Stanek; Gordon Winter; Lujuan Xu; Christian Laube; Tanja Weil; Volker Rasche; Claudia Kuntner

doi:10.1016/j.nucmedbio.2022.108310

Synthesis, radiolabeling, and preclinical in vivo evaluation of ⁶⁸Ga-radiolabelled nanodiamonds

Nucl Med Biol. 2023 Jan-Feb:116-117:108310. doi: 10.1016/j.nucmedbio.2022.108310. Epub 2022 Dec 20.

Authors

Affiliations

¹ Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria; Preclinical Molecular Imaging, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
² Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria; Preclinical Molecular Imaging, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria; Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria.
³ Max Planck Institute for Polymer Research, Synthesis of Macromolecules, Mainz, Germany; Institute of Inorganic Chemistry I, Ulm University, Ulm, Germany.
⁴ Preclinical Molecular Imaging, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria; Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria.
⁵ Department of Nuclear Medicine, Ulm University Medical Center, Ulm, Germany(.).
⁶ Leibniz-Institute of Surface Engineering (IOM), Leipzig, Germany.
⁷ Core Facility Small Animal Imaging, Ulm University, Ulm, Germany.
⁸ Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria; Preclinical Molecular Imaging, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria. Electronic address: claudia.kuntner@meduniwien.ac.at.

PMID: 36565646
DOI: 10.1016/j.nucmedbio.2022.108310

Abstract

Purpose: Nanodiamonds (NDs) represent a new class of nanoparticles and have gained increasing interest in medical applications. Modifying the surface coating by attaching binding ligands or imaging probes can transform NDs into multi-modal targeting probes. This study evaluated the biokinetics and biodistribution of ⁶⁸Ga-radiolabelled NDs in a xenograft model.

Procedures: NDs were coated with an albumin-derived copolymer modified with desferrioxamine to provide a chelator for radiolabeling. In vivo studies were conducted in AR42J tumor-bearing CD1 mice to evaluate biodistribution and tumor accumulation of the NDs.

Results: Coated NDs were successfully radiolabeled using ⁶⁸Ga at room temperature with radiolabeling efficiencies up to 91.8 ± 3.2 % as assessed by radio-TLC. In vivo studies revealed the highest accumulation in the liver and spleen, whereas tumor radioactivity concentration was low.

Conclusions: Radiolabeling of coated NDs could be achieved. However, the obtained results indicate these coated NDs' limitations in their biodistribution within the conducted studies.

Keywords: Nanodiamonds; Radiolabeling; Small animal PET.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gallium Radioisotopes
Humans
Mice
Nanodiamonds*
Neoplasms*
Polymers
Tissue Distribution

Substances

Nanodiamonds
Gallium Radioisotopes
Polymers