A Lentiviral Pseudotype System to Characterize SARS-CoV-2 Glycoprotein

Methods Mol Biol. 2023:2610:187-199. doi: 10.1007/978-1-0716-2895-9_16.

Abstract

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 causes worldwide COVID-19 pandemic and poses a great threat to global public health. Due to its high pathogenicity and infectivity, live SARS-CoV-2 is classified as a BSL-3 agent and has to be handled in BSL-3 condition. Nevertheless, entry of SARS-CoV-2 is mediated by viral spike (S) glycoprotein, and pseudovirus with SARS-CoV-2 S protein can mimic every entry step of SARS-CoV-2 virus and be studied in BSL-2 settings. In this chapter, we describe a detailed protocol of production of lentivirus-based SARS-CoV-2 S pseudovirus and its application in study of virus entry and determination of neutralizing antibody titer of human sera against SARS-CoV-2.

Keywords: Lentiviral pseudotype; Neutralization assay; SARS-CoV-2; Spike protein; Virus entry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • COVID-19*
  • Glycoproteins
  • Humans
  • Neutralization Tests / methods
  • Pandemics
  • SARS-CoV-2* / metabolism
  • Spike Glycoprotein, Coronavirus

Substances

  • spike protein, SARS-CoV-2
  • Antibodies, Viral
  • Antibodies, Neutralizing
  • Spike Glycoprotein, Coronavirus
  • Glycoproteins