Characterization of SARS-CoV-2 Glycoprotein Using a Quantitative Cell-Cell Fusion System

Xiuyuan Ou; Zhaohui Qian

doi:10.1007/978-1-0716-2895-9_15

Characterization of SARS-CoV-2 Glycoprotein Using a Quantitative Cell-Cell Fusion System

Methods Mol Biol. 2023:2610:179-186. doi: 10.1007/978-1-0716-2895-9_15.

Authors

Xiuyuan Ou¹, Zhaohui Qian²

Affiliations

¹ NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. zqian2013@sina.com.

PMID: 36534291
DOI: 10.1007/978-1-0716-2895-9_15

Abstract

Coronaviruses (CoVs) infect host cells through the fusion of viral and cellular membrane and may also spread to the neighboring uninfected cells from infected cells through cell-cell fusion. The viral spike (S) glycoproteins play an essential role in mediating membrane fusion. Here, we present a luciferase-based quantitative assay to measure the efficiency of cell-cell fusion mediated by the S protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This method applies to S proteins of the other coronaviruses and can be adapted to fusion proteins of other enveloped viruses.

Keywords: Cell; Cell fusion; Coronavirus; Human angiotensin-converting enzyme 2; Luciferase; SARS-CoV-2; Spike glycoprotein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19*
Cell Fusion
Glycoproteins
Humans
SARS-CoV-2* / metabolism
Spike Glycoprotein, Coronavirus / metabolism
Virus Internalization

Substances

Glycoproteins
Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2