Cytotoxic steroidal saponins from the rhizomes of Paris fargesii var. Petiolata

Abstract

Phytochemical investigation on the rhizomes of Paris fargesii var. petiolata (Baker ex C. H. Wright) Wang et Tang led to the isolation of five previously undescribed steroidal saponins, parpetiosides A-E (1-5), and six known analogs (6-11). Their structures were established by extensive spectroscopic data analysis and chemical methods. Compound 5 was a rare steroidal saponin with disaccharide moiety linked at C-26 of dehydrokryptogenin that was hardly seen in the genus Paris. The cytotoxicities of the isolated compounds against three human cancer cell lines (U87, HepG2 and SGC-7901) were evaluated, and compound 1 displayed certain inhibitory effect with IC₅₀ values of 8.02 ± 0.45, 8.24 ± 0.57 and 6.20 ± 0.79 μM, respectively. Moreover, the preliminary mechanism of 1 inhibiting the proliferation of the three cancer cell lines might be related to cell cycle distribution and the induction of S phase arrest.

Keywords: Cell cycle arrest; Cholestanol-type saponins; Cytotoxic; Furostanol-type saponins; Isospirostanol-type saponins; Paris fargesii var. petiolata.