SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection

Exp Mol Med. 2022 Dec;54(12):2188-2199. doi: 10.1038/s12276-022-00891-0. Epub 2022 Dec 9.

Abstract

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems* / genetics
  • Gene Knockout Techniques
  • Introns / genetics
  • Mice
  • Zygote*