FeCl3 and Fe2(SO4)3 differentially reduce Cd uptake and accumulation in Polish wheat (Triticum polonicum L.) seedlings by exporting Cd from roots and limiting Cd binding in the root cell walls

Abstract

Wheat grown in cadmium (Cd)-contaminated soils easily accumulates more Cd in edible parts than the Chinese safety limit (0.1 mg/kg). FeCl₃ and Fe₂(SO₄)₃ have been used to extract Cd from Cd-contaminated soils. Thus, we hypothesized that FeCl₃ and Fe₂(SO₄)₃, used as iron (Fe) fertilizers, can reduce Cd uptake and accumulation in wheat. Here, a hydroponic experiment was performed with three FeCl₃ and Fe₂(SO₄)₃ concentrations under 80 μM CdCl₂ stress on dwarf Polish wheat (Triticum polonicum L., 2n = 4x = 28, AABB) seedlings. Compared with Fe deficiency, FeCl₃ and Fe₂(SO₄)₃ additions competitively reduced Cd concentrations. The reductions were not associated with changes in dry weight and root morphological parameters. FeCl₃ and Fe₂(SO₄)₃ additions reduced Cd concentrations in the following order from smallest to largest reduction: 25 μM Fe₂(SO₄)₃ < 200 μM FeCl₃ < 50 μM FeCl₃ < 100 μM Fe₂(SO₄)₃. Investigation of subcellular distributions showed that the four Fe fertilizers differentially reduced Cd binding in the root cell walls and enhanced root sucrose and trehalose. Cd chemical form analysis revealed that Fe fertilizer addition also differentially reduced root F_E, F_W, and F_NaCl. Transcriptomic analysis revealed that addition of FeCl₃ and Fe₂(SO₄)₃ differentially up-regulated several genes that hydrolyze cell wall polysaccharides and metal transporter genes for Cd uptake (IRT1 and CAX19) and export (ZIP1, ABCG11, ABCG14, ABCG28, ABCG37, ABCG44, and ABCG48) reducing Cd uptake and accumulation. Our results demonstrated that FeCl₃ and Fe₂(SO₄)₃ can reduce Cd accumulation in wheat, and 50 μM FeCl₃ is the most effective treatment.

Keywords: Cadmium; Dwarf Polish wheat; Iron fertilizer; Metal transporters; Subcelluar distribution.