Comparative evaluation of incorporation calcium silicate and calcium phosphate nanoparticles on biomimetic dentin remineralization and bioactivity in an etch-and-rinse adhesive system

Zohre Ashtijoo; Leila Pishevar; Mohammad-Reza MalekipourMalekipour; Mohammad Khodaei; Zahra Sabouri

doi:10.4317/jced.59817

Comparative evaluation of incorporation calcium silicate and calcium phosphate nanoparticles on biomimetic dentin remineralization and bioactivity in an etch-and-rinse adhesive system

J Clin Exp Dent. 2022 Nov 1;14(11):e903-e910. doi: 10.4317/jced.59817. eCollection 2022 Nov.

Authors

Zohre Ashtijoo¹, Leila Pishevar², Mohammad-Reza MalekipourMalekipour³, Mohammad Khodaei⁴, Zahra Sabouri⁵

Affiliations

¹ Private Dentist, Department of Operative Dentistry, Faculty of Dentistry, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran.
² Assistant Professor, Department of Operative Dentistry, Faculty of Dentistry, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran.
³ Associated Professor, Department of Operative Dentistry, Faculty of Dentistry, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran.
⁴ Materials Engineering Group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan, 87717-67498, IranMaterials Engineering Group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan, 87717-67498, Iran.
⁵ Department of Materials Engineering, Isfahan University of Technology, Isfahan84156-83111, Iran.

Abstract

Background: This study aimed to evaluate the remineralization potential and bioactivity of adhesives, containing amorphous calcium phosphate (ACP) and calcium silicate (CS) nanoparticles (NPs).

Material and methods: In this study, dentin slices (n=60) were prepared and etched with phosphoric acid. Next, they were divided into two groups: pre- and post-immersion in a simulated body fluid (SBF) for three weeks. The two groups were also divided into five subgroups (n=6 per subgroup), including the control (0 wt.% NPs); adhesives containing 1 wt.% and 2.5 wt.% (CS) nanoparticles; and adhesives containing 1 wt.% and 2.5 wt.% ACP nanoparticles. The remineralization potential and bioactivity of the adhesives were evaluated. The shear bond strength of the samples (n=18) was also assessed using a universal testing machine.

Results: The present results revealed that the adhesive containing ACP and CS nanoparticles showed bioactivity and remineralization potential without any reduction in the bond strength.

Conclusions: The outcomes revealed that Cs and ACP nanoparticles induced mineralization in the dentin and incorporation of these nanoparticles to dentin bonding agents could improve the bio-functionalization of dentin bond. Key words:Calcium phosphate, calcium silicate, fourier transform infrared spectroscopy, scanning electron microscopy, tooth remineralization.