Defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress: A contradiction between attraction and resistance

Abstract

Heavy metal pollution, as a common and serious environmental problem worldwide, has been regarded as an abiotic stimulus that can affect plant insect resistance and pest occurrence. This study evaluated the defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress, with consideration given to chemical defense, physical defense, and elemental defense. Our results showed that the H. cunea larvae had a strong preference for Cd-treated F. mandshurica seedlings, but there was a significant reduction in body weight and survival rate in larvae that fed on leaves of Cd-treated seedlings. Under Cd treatment, the increase in attractant metabolites (e.g., styrene, dibutyl phthalate, and d-limonene) and the decrease in repellent metabolites (e.g., aromadendrene, heptadecane, and camphene) in leaf volatiles were responsible for the high attractant activity to H. cunea larvae. Based on leaf physicochemical properties, tissue structure, and phenolic acid content, an overall reduction in physical defense, chemical defense and their combination in F. mandshurica seedlings exposed to Cd stress was identified by Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) analysis. Elemental defense occurred in Cd-treated F. mandshurica seedlings, as evidenced by the high concentration of Cd in leaves and H. cunea larvae under Cd treatment. Taken together, these findings demonstrate that under Cd stress, elemental defense replaces the dominant role of basic defense in F. mandshurica seedlings and accounts for the enhanced ability to defend against H. cunea larvae.

Keywords: Chemical defense; Elemental defense; Food chain; Heavy metal; Physical defense.