Effect of homemade compound microbial inoculum on the reduction of terramycin and antibiotic resistance genes in terramycin mycelial dreg aerobic composting and its mechanism

Sainan Chen; Weizhang Zhong; Zhifang Ning; Jianrui Niu; Jing Feng; Xue Qin; Zaixing Li

doi:10.1016/j.biortech.2022.128302

Effect of homemade compound microbial inoculum on the reduction of terramycin and antibiotic resistance genes in terramycin mycelial dreg aerobic composting and its mechanism

Bioresour Technol. 2023 Jan:368:128302. doi: 10.1016/j.biortech.2022.128302. Epub 2022 Nov 18.

Authors

Sainan Chen¹, Weizhang Zhong², Zhifang Ning¹, Jianrui Niu¹, Jing Feng³, Xue Qin¹, Zaixing Li¹

Affiliations

¹ College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; Pollution Prevention Biotechnology Laboratory of Hebei Province, Shijiazhuang 050018, China.
² College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; Pollution Prevention Biotechnology Laboratory of Hebei Province, Shijiazhuang 050018, China. Electronic address: zhongweizhang@hebust.edu.cn.
³ Key Laboratory of Energy Resource Utilization from Agricultural Residues, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Agricultural Planning and Engineering, Beijing 100125, China.

PMID: 36403916
DOI: 10.1016/j.biortech.2022.128302

Abstract

In order to tackle the issue of terramycin mycelial dreg (TMD) diagnosis and removal of terramycin and antibiotic resistance genes (ARGs), this study adopted aerobic composting (AC) technology and added homemade compound microbial inoculum (HCMI) to promote the AC of TMD and enhance the removal of terramycin and ARGs. The findings demonstrated that terramycin residue could be basically harmless after AC. Moreover, HCMI not only reduced QacB and tetH but also increased the degradation rates of VanRA, VanT, and dfrA24 by 40.81%, 5.65%, and 54.18%, respectively. The HCMI improved the removal rate of ARG subtypes to a certain extent. According to redundancy analysis, during AC, the succession of the microbial community had a stronger influence on the variance of ARG subtype than the environmental conditions. Differences in the abundance of various bacteria due to changes in temperature may be an intrinsic mechanism for the variation of ARG subtypes.

Keywords: Antibiotic mycelial dreg; Antibiotic residue; Co-composting; Metagenomics; Microbial community.

MeSH terms

Anti-Bacterial Agents / pharmacology
Composting*
Drug Resistance, Microbial / genetics
Mycelium
Oxytetracycline*

Substances

Oxytetracycline
Anti-Bacterial Agents