Transcriptome analysis of gene expression in the tomato leaf premature senescence mutant

Fengmei Jin; Mingyan Hua; Lanfang Song; Shaojie Cui; Haibo Sun; Weidong Kong; Zhiyu Hao

doi:10.1007/s12298-022-01223-2

Transcriptome analysis of gene expression in the tomato leaf premature senescence mutant

Physiol Mol Biol Plants. 2022 Aug;28(8):1501-1513. doi: 10.1007/s12298-022-01223-2. Epub 2022 Aug 28.

Authors

Fengmei Jin¹, Mingyan Hua¹, Lanfang Song¹, Shaojie Cui¹, Haibo Sun¹, Weidong Kong¹, Zhiyu Hao¹

Affiliation

¹ Tianjin Academy of Agriculture Sciences, Tianjin, 300192 China.

Abstract

Premature senescence of leaves can critically influence tomato yield and quality. In this study, the leaf premature senescence mutant MT318 was a spontaneous mutant and was controlled by a single recessive nuclear gene. The maximum photochemical efficiency (Fv/Fm), superoxide dismutase (SOD), and chlorophyll content in the leaves of mutant MT318 gradually decreased, while malondialdehyde (MDA) content significantly increased. Under the level 2 category, Gene Ontology (GO) enrichment analysis indicated that 45 terms were enriched, comprising 22 in biological process, 12 in cellular component, and 11 in molecular function. Genes are mainly involved in the metabolic processes (696 differentially expressed genes, DEGs), cellular processes (573 DEGs), single-organism processes (503 DEGs), and catalytic activity (675 DEGs). Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that the 4 pathways with the largest number of genes were biosynthesis of secondary metabolites, plant-pathogen interaction, plant hormone signal transduction, and MAPK signaling pathway-plant. The 'plant hormone signal transduction' pathway was the most significantly enriched at the T2 stage. Pearson correlation analysis showed that the auxin regulatory pathway and SA signal transduction pathway may play important roles. These results not only lay the foundation for the further cloning and functional analysis of the MT318 premature senescence gene but also provide a reference for the study of tomato leaf senescence.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-022-01223-2.

Keywords: Mutant; RNA-Seq; Senescence; Solanum lycopersicum.

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