Urinary concentrations of fungicide carbendazim's metabolite and associations with oxidative stress biomarkers in young children

Abstract

Carbendazim (CBDZ) is the most widely used fungicide in China. It is ubiquitous in environment and can induce oxidative stress in mammals, while data on occurrence of its metabolite in human urine are scarce, and the relationship between CBDZ and oxidative stress biomarkers (OSBs) in young children has not been examined. The aim of this study was to measure the concentrations of methyl 5-hydroxy-2-benzimidazolecarbamate (5-HBC, the main metabolite of CBDZ in urine) in 390 urine samples collected from 130 healthy young (< 6.6 years old) children from Shenzhen and Wuhan, in south and central China, respectively, and to evaluate the associations of 5-HBC with three selected OSBs (4-HNEMA, 8-OHG, and 8-OHdG, for lipid, RNA, and DNA, respectively). 5-HBC was found in 99.2% of the urine samples at concentrations ranging from below the method detection limit (< 0.005 ng/mL) to 10.9 ng/mL (median: 0.11 ng/mL). Moderate inter-day reproducibility was found for specific gravity-adjusted 5-HBC concentrations (intraclass correlation coefficient: 0.50). The urinary 5-HBC concentrations were significantly and positively associated with 4-HNEMA (p < 0.01). An interquartile range increase in urinary 5-HBC concentrations was associated with a 42.1% increase in 4-HNEMA, which implied that CBDZ exposure might be associated with lipid peroxidation in young children without occupational exposure. As far as we know, this pilot study is the first to report urinary 5-HBC and its associations with OSBs in children.

Keywords: Carbendazim; Methyl 5-hydroxy-2-benzimidazolecarbamate; Oxidative stress biomarker; Urine; Young children.