Effects of ginkgolic acid(C15:1)on biofilm formation, pathogenic factor production and quorum sensing of Pseudomonas aeruginosa

Andi Suo; Zhebin Hua; Caie Wu; Gongjian Fan; Tingting Li; Kaiping Cong

doi:10.1016/j.micpath.2022.105813

Effects of ginkgolic acid(C15:1)on biofilm formation, pathogenic factor production and quorum sensing of Pseudomonas aeruginosa

Microb Pathog. 2022 Dec;173(Pt A):105813. doi: 10.1016/j.micpath.2022.105813. Epub 2022 Oct 3.

Authors

Andi Suo¹, Zhebin Hua², Caie Wu³, Gongjian Fan⁴, Tingting Li⁴, Kaiping Cong¹

Affiliations

¹ College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
² Graduate Student Office, Nanjing Institute of Technology, Nanjing, 210037, Jiangsu, China.
³ College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China. Electronic address: wucaie@njfu.edu.cn.
⁴ College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.

PMID: 36202349
DOI: 10.1016/j.micpath.2022.105813

Abstract

To complete the investigation of the bacterial inhibitory activity of ginkgolic acid (GA), the quorum sensing activity of GA was investigated to assess the potential of ginkgo biloba exocarp for the production of quorum sensing inhibitor. The effect of GA (C15:1) on the growth and secondary metabolism of p. aeruginosa PAO1 were tested. The results showed that GA (C15:1) had no effect on the growth of p. aeruginosa PAO1, and that GA (C15:1) was able to inhibit the formation of quorum sensing dependent virulence factors, but promoted the formation of biofilms. Finally, the above experimental results were verified by LasR-GFP, RhlR-GFP system and fluorescence quantitative PCR. These results suggested that GA (C15:1) was capable of modulating the quorum sensing system of p. aeruginosa PAO1 and possessed potential as an anti-virulence factor drug, but is not suitable for development as a broad-spectrum biofilm inhibitor.

Keywords: Biofilm formation; Ginkgolic acid; Pseudomonas aeruginosa; Pyocyanin production; Quorum sensing.

MeSH terms

Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / pharmacology
Biofilms
Pseudomonas aeruginosa*
Quorum Sensing*
Virulence Factors

Substances

Virulence Factors
ginkgolic acid
Anti-Bacterial Agents
Bacterial Proteins