Tumor Microenvironment Multiple Responsive Nanoparticles for Targeted Delivery of Doxorubicin and CpG Against Triple-Negative Breast Cancer

Int J Nanomedicine. 2022 Sep 20:17:4401-4417. doi: 10.2147/IJN.S377702. eCollection 2022.

Abstract

Introduction: Currently, the main treatment for advanced breast cancer is still chemotherapy. Immunological and chemical combination therapy has a coordinated therapeutic effect and achieves some efficacy. However, the immunosuppressive tumor microenvironment is a major cause for the failure of immunotherapy in breast cancer. CpG oligodeoxynucleotides can activate the tumor immune microenvironment to reverse the failure of immunotherapy.

Methods: In this study, we designed an amphiphilic peptide micelle system (Co-LMs), which can targeted delivery of the immune adjuvant CpG and the chemotherapeutic drug doxorubicin to breast cancer tumors simultaneously. The peptide micelle system achieved tumor microenvironment pH and redox-sensitive drug release.

Results and discussion: Co-LMs showed 2.3 times the antitumor efficacy of chemotherapy alone and 5.1 times the antitumor efficacy of immunotherapy alone in triple-negative breast cancer mice. Co-LMs activated cytotoxic CD8+ T lymphocytes and CD4+ T cells in mice to a greater extent than single treatments. We also found that Co-LMs inhibited the metastasis of circulating tumor cells in the bloodstream to some extent. These results indicate that the Co-LMs offer a promising therapeutic strategy against triple-negative breast cancer.

Keywords: CpG; chemo-immunotherapy; nanoparticles; triple-negative breast cancer; tumor microenvironment.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Doxorubicin / pharmacology
  • Doxorubicin / therapeutic use
  • Humans
  • Immunotherapy
  • Mice
  • Micelles
  • Nanoparticles*
  • Oligodeoxyribonucleotides / pharmacology
  • Peptides / pharmacology
  • Triple Negative Breast Neoplasms* / pathology
  • Tumor Microenvironment

Substances

  • Micelles
  • Oligodeoxyribonucleotides
  • Peptides
  • Doxorubicin

Grants and funding

This work was funded by the National Natural Science Foundation of China (Nos. NSFC81972891); the Science and Technology Project of Jiaxing, Zhejiang, China (Grants 2019AY32013); the Research Fund for Academician Lin He New Medicine (Grants199331309) and the Foundation of Shanghai Pharmaceutical Association.